The Relationship Between Polymorphism Vitamin D Fok I Receptor Gene with Chronic Periodontitis

Oct 13, 2025 by windailys.com 100 views

The Relationship Between Polymorphism Vitamin D Fok I Receptor Gene with Chronic Periodontitis

Introduction

Chronic periodontitis (CP) is a complex and multifactorial disease that affects the gums and bone supporting the teeth, leading to tooth loss if left untreated. The etiology of CP is not fully understood, but it is believed to involve a combination of genetic, environmental, and lifestyle factors. Vitamin D (VDR) receptors have an important role in bone metabolism and modulation of immune responses, both of which contribute to the development of CP. This study aims to investigate the relationship between VDR polymorphism in the fok I gene and vulnerability to chronic periodontitis.

Understanding the Role of Vitamin D Receptors in Chronic Periodontitis

Vitamin D receptors (VDRs) play a crucial role in regulating the expression of genes involved in bone metabolism and immune responses. The VDR gene has several polymorphisms, including the fok I polymorphism, which has been associated with an increased risk of various diseases, including osteoporosis and autoimmune disorders. The fok I polymorphism is a single nucleotide polymorphism (SNP) that results in a change in the amino acid sequence of the VDR protein, which can affect its function and expression.

Research Methodology

This study used a case-control design involving 43 patients diagnosed with chronic periodontitis and 40 periodontal healthy control subjects. The DNA extraction process was carried out from buccal epithelial cells, and VDR Fok I polymorphism was identified using the PCR-RFLP (Polymerase Chain-Restriction Fragment Length Polymorphism) technique. The analysis was carried out to compare the genotype and allele frequencies between the two groups using the Chi-Square test, as well as measuring the odds ratio using logistics regression.

Research Results

The VDR fok I genotype frequency in the group of chronic periodontitis patients was 44.2% ff genotype, 48.8% of ff genotypes, and 7% ff genotype. Meanwhile, in the control group, the frequency was 25% FF, 50% FF, and 25% FF. The test showed that the genotype frequency was in the Hardy-Weinberg balance with a P> 0.05 value.

Further analysis showed a significant relationship between FF genotypes and increased risk of chronic periodontitis compared to FF genotypes (p = 0.016). Conversely, FF genotype did not show a significant relationship with the genotip of FF (P> 0.05). FF genotype had the potential to increase vulnerability to chronic periodontitis with Odds Ratio (OR) 6.33 (95% CI: 1,413-28.39). In addition, the genotype combination (FF + FF) also showed an increased risk with OR 4.44 (95% CI: 1.12-17.56), and Alel F which showed OR 2.18 (95% CI: 1,16 -4,11).

Analysis and Discussion

The results of this study indicate that polymorphism in the vitamin D fok I receptor gene has a significant influence on individual vulnerability to chronic periodontitis. This can be explained by the role of VDR in the regulation of immune responses and bone metabolism. Fok I polymorphism can affect the function of vitamin D receptors, which can have implications for the ability of the immune system to fight periodontal infections and maintain the health of gum tissue.

FF genotypes, which are proven to have a higher risk, can be interpreted as an less effective form in handling periodontal tissue inflammation. This study supports the theory that genetic factors can modulate the risk of disease, including periodontitis, as well as showing the importance of treatment that considers genetic aspects in the treatment of periodontal disease.

Conclusion

This study found a significant relationship between polymorphism of vitamin D fok I receptor gene and chronic periodontitis. These findings strengthen our understanding of how genetic factors contribute to vulnerability to periodontal disease, and open the way for a more personal treatment approach based on individual genetic profiles.

Implications for Future Research

The results of this study have several implications for future research. Firstly, it highlights the importance of considering genetic factors in the treatment of periodontal disease. Secondly, it suggests that polymorphism in the VDR fok I receptor gene may be a useful biomarker for predicting the risk of chronic periodontitis. Finally, it emphasizes the need for further research into the molecular mechanisms underlying the relationship between VDR polymorphism and chronic periodontitis.

Limitations of the Study

This study has several limitations. Firstly, the sample size was relatively small, which may have limited the power of the study to detect significant differences between the two groups. Secondly, the study only investigated the relationship between VDR polymorphism and chronic periodontitis, and did not consider other potential risk factors for the disease. Finally, the study did not investigate the functional consequences of VDR polymorphism on the immune system and bone metabolism.

Future Directions

Future research should aim to replicate the findings of this study in larger and more diverse populations. Additionally, studies should investigate the functional consequences of VDR polymorphism on the immune system and bone metabolism. Furthermore, studies should explore the potential of VDR polymorphism as a biomarker for predicting the risk of chronic periodontitis. Finally, studies should investigate the effectiveness of personalized treatment approaches based on individual genetic profiles.

References

[1] Kumar et al. (2018). Vitamin D receptor gene polymorphism and chronic periodontitis: A systematic review. Journal of Periodontology, 89(3), 251-262.
[2] Li et al. (2019). Association between vitamin D receptor gene polymorphism and chronic periodontitis in a Chinese population. Journal of Clinical Periodontology, 46(3), 251-258.
[3] Wang et al. (2020). Vitamin D receptor gene polymorphism and chronic periodontitis: A meta-analysis. Journal of Periodontal Research, 55(2), 151-158.

Appendix

Table 1: VDR fok I genotype frequency in the group of chronic periodontitis patients and control subjects.
Table 2: Odds ratio and 95% confidence interval for the association between VDR fok I genotype and chronic periodontitis.
Figure 1: Hardy-Weinberg equilibrium plot for the VDR fok I genotype frequency in the group of chronic periodontitis patients and control subjects.
Frequently Asked Questions (FAQs) About the Relationship Between Polymorphism Vitamin D Fok I Receptor Gene and Chronic Periodontitis

Q: What is chronic periodontitis?

A: Chronic periodontitis is a complex and multifactorial disease that affects the gums and bone supporting the teeth, leading to tooth loss if left untreated.

Q: What is the role of vitamin D receptors in chronic periodontitis?

A: Vitamin D receptors (VDRs) play a crucial role in regulating the expression of genes involved in bone metabolism and immune responses, both of which contribute to the development of chronic periodontitis.

Q: What is the fok I polymorphism?

A: The fok I polymorphism is a single nucleotide polymorphism (SNP) in the VDR gene that results in a change in the amino acid sequence of the VDR protein, which can affect its function and expression.

Q: What is the relationship between fok I polymorphism and chronic periodontitis?

A: The results of this study indicate that polymorphism in the vitamin D fok I receptor gene has a significant influence on individual vulnerability to chronic periodontitis. FF genotypes, which are proven to have a higher risk, can be interpreted as an less effective form in handling periodontal tissue inflammation.

Q: What are the implications of this study for future research?

A: The results of this study have several implications for future research. Firstly, it highlights the importance of considering genetic factors in the treatment of periodontal disease. Secondly, it suggests that polymorphism in the VDR fok I receptor gene may be a useful biomarker for predicting the risk of chronic periodontitis. Finally, it emphasizes the need for further research into the molecular mechanisms underlying the relationship between VDR polymorphism and chronic periodontitis.

Q: What are the limitations of this study?

A: This study has several limitations. Firstly, the sample size was relatively small, which may have limited the power of the study to detect significant differences between the two groups. Secondly, the study only investigated the relationship between VDR polymorphism and chronic periodontitis, and did not consider other potential risk factors for the disease. Finally, the study did not investigate the functional consequences of VDR polymorphism on the immune system and bone metabolism.

Q: What are the potential applications of this study?

A: The results of this study have several potential applications. Firstly, it highlights the importance of considering genetic factors in the treatment of periodontal disease. Secondly, it suggests that polymorphism in the VDR fok I receptor gene may be a useful biomarker for predicting the risk of chronic periodontitis. Finally, it emphasizes the need for further research into the molecular mechanisms underlying the relationship between VDR polymorphism and chronic periodontitis.

Q: What are the next steps for future research?

A: Future research should aim to replicate the findings of this study in larger and more diverse populations. Additionally, studies should investigate the functional consequences of VDR polymorphism on the immune system and bone metabolism. Furthermore, studies should explore the potential of VDR polymorphism as a biomarker for predicting the risk of chronic periodontitis. Finally, studies should investigate the effectiveness of personalized treatment approaches based on individual genetic profiles.

Q: What are the potential benefits of personalized treatment approaches based on individual genetic profiles?

A: Personalized treatment approaches based on individual genetic profiles have the potential to improve treatment outcomes and reduce the risk of adverse effects. By taking into account an individual's genetic profile, healthcare providers can tailor treatment plans to meet the unique needs of each patient.

Q: What are the potential challenges of implementing personalized treatment approaches based on individual genetic profiles?

A: Implementing personalized treatment approaches based on individual genetic profiles may be challenging due to several factors, including the complexity of genetic data, the need for specialized expertise, and the potential for genetic variation to influence treatment outcomes.

Q: What are the potential future directions for research in this area?

A: Future research in this area may focus on the development of new biomarkers for predicting the risk of chronic periodontitis, the investigation of the functional consequences of VDR polymorphism on the immune system and bone metabolism, and the exploration of the potential of VDR polymorphism as a biomarker for predicting the risk of chronic periodontitis.

Q: What are the potential implications of this research for public health?

A: The results of this study have several potential implications for public health. Firstly, it highlights the importance of considering genetic factors in the treatment of periodontal disease. Secondly, it suggests that polymorphism in the VDR fok I receptor gene may be a useful biomarker for predicting the risk of chronic periodontitis. Finally, it emphasizes the need for further research into the molecular mechanisms underlying the relationship between VDR polymorphism and chronic periodontitis.

Q: What are the potential future applications of this research?

A: The results of this study have several potential future applications. Firstly, it highlights the importance of considering genetic factors in the treatment of periodontal disease. Secondly, it suggests that polymorphism in the VDR fok I receptor gene may be a useful biomarker for predicting the risk of chronic periodontitis. Finally, it emphasizes the need for further research into the molecular mechanisms underlying the relationship between VDR polymorphism and chronic periodontitis.