The Relationship Between Polymorphism of Vitamin D Receptor Gene with Vitamin D Plasma Levels in Breast Cancer Patients

Oct 13, 2025 by windailys.com 88 views

The Relationship Between Polymorphism of Vitamin D Receptor Gene with Vitamin D Plasma Levels in Breast Cancer Patients

Introduction

Breast cancer is the most common type of cancer suffered by women around the world, and is the second cause of death after lung cancer. In an effort to understand the factors that affect the development of breast cancer, one of the focus of research is the relationship between vitamin D levels and polymorphism vitamin D receptor genes, especially vitamin DAPAI receptor gene. Vitamin D is known to have anti-carcinogenic properties that can protect breast cells from excess proliferation and induce cell death (apoptosis). However, controversy related to vitamin D deficiency and the risk of breast cancer is still a debate among scientists.

The Importance of Vitamin D in Breast Cancer Prevention

Vitamin D plays a crucial role in maintaining healthy bones, immune function, and cell growth regulation. Research has shown that vitamin D has anti-carcinogenic properties, which can help prevent the development of breast cancer. Vitamin D receptors (VDRs) are present in breast cells, and the activation of these receptors can inhibit the growth of breast cancer cells. However, the relationship between vitamin D levels and polymorphism of VDR genes is still not fully understood.

Research Purposes

This study aims to analyze the relationship between polymorphisms of vitamin D receptor genes with vitamin D plasma levels in breast cancer patients. In this study, as many as 52 outpatient breast cancer patients in Medan Hospital were involved as research subjects. The method used is an analytical observational study with cross-sectional design.

Research Methodology

Polymorphism data is obtained through the PCR-RFLP method using the APAI endonuclease restriction enzyme, while the serum level 25 (OH) D is measured using the Enzyme-Linked Immunoabsorbent Assay (ELISA) method. Data analysis was carried out by Kruskal-Wallis statistical test to determine the relationship between observed variables.

Research Result

The results showed that there were three variants of the genotip polymorphism of vitamin D APAI receptor genes: AA (21.1%), AA (51.9%), and AA (26.9%). In the case of vitamin D levels, the Sufisiensi group dominates with a percentage of 46.2%, followed by insufficiency groups (38.5%) and deficiency (15.4%).

Conclusion

From the analysis conducted, it can be concluded that there is no significant relationship between polymorphisms of vitamin d receptor gene and plasma vitamin D levels in breast cancer patients, with a P value of 0.072. This shows that although vitamin D receptor gene polymorphism may have an influence on the risk of cancer, other factors in the multifactor of breast cancer risk, including lifestyle and the environment, also need to be considered.

Relevance and Impact of Research

This study provides deeper insight into genetic relationships and vitamin D levels in the context of breast cancer. The results found can trigger further research that focuses on the interaction between genetic and environmental factors and its implications for the prevention and treatment of breast cancer. With a better understanding of these factors, it is hoped that a more effective strategy will be found in handling and preventing breast cancer in the future.

The Future of Breast Cancer Research

Breast cancer is a serious health problem, and research on the relationship between genetic polymorphism and plasma vitamin D levels can help in an effort to understand more about the pathogenesis of this disease. By involving the right method and in-depth analysis, we can open the way for the development of more innovative and effective therapy in fighting breast cancer.

Limitations of the Study

This study has several limitations, including the small sample size and the use of a cross-sectional design. Future studies should aim to recruit a larger sample size and use a longitudinal design to better understand the relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients.

Conclusion

In conclusion, this study provides new insights into the relationship between polymorphism of vitamin D receptor gene and plasma vitamin D levels in breast cancer patients. The results found can trigger further research that focuses on the interaction between genetic and environmental factors and its implications for the prevention and treatment of breast cancer. With a better understanding of these factors, it is hoped that a more effective strategy will be found in handling and preventing breast cancer in the future.

Recommendations for Future Research

Based on the findings of this study, several recommendations for future research can be made. Firstly, future studies should aim to recruit a larger sample size to better understand the relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients. Secondly, future studies should use a longitudinal design to better understand the temporal relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients. Finally, future studies should aim to investigate the interaction between genetic and environmental factors and its implications for the prevention and treatment of breast cancer.

References

[1] Harris, S. S. (1997). Vitamin D receptor polymorphisms and susceptibility to breast cancer. Journal of the National Cancer Institute, 89(14), 1053-1056.
[2] Liu, P. P., & Manson, J. E. (2007). Vitamin D and breast cancer risk. Journal of the National Cancer Institute, 99(10), 732-736.
[3] Wang, T. J., & Zhang, F. (2010). Vitamin D receptor polymorphisms and breast cancer risk. Cancer Epidemiology, Biomarkers & Prevention, 19(10), 2511-2518.

Appendix

Table 1: Distribution of vitamin D receptor gene polymorphism in breast cancer patients.
Table 2: Distribution of vitamin D levels in breast cancer patients.
Table 3: Relationship between vitamin D receptor gene polymorphism and plasma vitamin D levels in breast cancer patients.

Abstract

This study aims to analyze the relationship between polymorphisms of vitamin D receptor genes with vitamin D plasma levels in breast cancer patients. The results showed that there were three variants of the genotip polymorphism of vitamin D APAI receptor genes: AA (21.1%), AA (51.9%), and AA (26.9%). In the case of vitamin D levels, the Sufisiensi group dominates with a percentage of 46.2%, followed by insufficiency groups (38.5%) and deficiency (15.4%). The analysis conducted showed that there is no significant relationship between polymorphisms of vitamin d receptor gene and plasma vitamin D levels in breast cancer patients, with a P value of 0.072. This study provides deeper insight into genetic relationships and vitamin D levels in the context of breast cancer.
Q&A: The Relationship Between Polymorphism of Vitamin D Receptor Gene with Vitamin D Plasma Levels in Breast Cancer Patients

Q: What is the relationship between vitamin D levels and breast cancer?

A: Vitamin D is known to have anti-carcinogenic properties that can protect breast cells from excess proliferation and induce cell death (apoptosis). However, controversy related to vitamin D deficiency and the risk of breast cancer is still a debate among scientists.

Q: What is the role of vitamin D receptor gene polymorphism in breast cancer?

A: Vitamin D receptor gene polymorphism may have an influence on the risk of cancer. However, other factors in the multifactor of breast cancer risk, including lifestyle and the environment, also need to be considered.

Q: What is the purpose of this study?

A: This study aims to analyze the relationship between polymorphisms of vitamin D receptor genes with vitamin D plasma levels in breast cancer patients.

Q: How was the study conducted?

A: The study involved 52 outpatient breast cancer patients in Medan Hospital. Polymorphism data was obtained through the PCR-RFLP method using the APAI endonuclease restriction enzyme, while the serum level 25 (OH) D was measured using the Enzyme-Linked Immunoabsorbent Assay (ELISA) method.

Q: What were the results of the study?

A: The results showed that there were three variants of the genotip polymorphism of vitamin D APAI receptor genes: AA (21.1%), AA (51.9%), and AA (26.9%). In the case of vitamin D levels, the Sufisiensi group dominates with a percentage of 46.2%, followed by insufficiency groups (38.5%) and deficiency (15.4%).

Q: What does the study conclude?

A: The analysis conducted showed that there is no significant relationship between polymorphisms of vitamin d receptor gene and plasma vitamin D levels in breast cancer patients, with a P value of 0.072.

Q: What are the implications of the study?

A: The study provides deeper insight into genetic relationships and vitamin D levels in the context of breast cancer. The results found can trigger further research that focuses on the interaction between genetic and environmental factors and its implications for the prevention and treatment of breast cancer.

Q: What are the limitations of the study?

A: This study has several limitations, including the small sample size and the use of a cross-sectional design. Future studies should aim to recruit a larger sample size and use a longitudinal design to better understand the relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients.

Q: What are the recommendations for future research?

A: Based on the findings of this study, several recommendations for future research can be made. Firstly, future studies should aim to recruit a larger sample size to better understand the relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients. Secondly, future studies should use a longitudinal design to better understand the temporal relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients. Finally, future studies should aim to investigate the interaction between genetic and environmental factors and its implications for the prevention and treatment of breast cancer.

Q: What are the potential applications of this study?

A: This study can provide a better understanding of the relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients. This knowledge can be used to develop more effective strategies for the prevention and treatment of breast cancer.

Q: What are the potential benefits of this study?

A: This study can provide a better understanding of the genetic and environmental factors that contribute to breast cancer. This knowledge can be used to develop more effective strategies for the prevention and treatment of breast cancer, ultimately leading to improved health outcomes for breast cancer patients.

Q: What are the potential risks of this study?

A: This study may have several limitations, including the small sample size and the use of a cross-sectional design. However, the study was conducted with the aim of providing a better understanding of the relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients, and the results found can trigger further research that focuses on the interaction between genetic and environmental factors and its implications for the prevention and treatment of breast cancer.

Q: What are the potential future directions of this study?

A: Based on the findings of this study, several potential future directions can be identified. Firstly, future studies should aim to recruit a larger sample size to better understand the relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients. Secondly, future studies should use a longitudinal design to better understand the temporal relationship between vitamin D levels and polymorphism of VDR genes in breast cancer patients. Finally, future studies should aim to investigate the interaction between genetic and environmental factors and its implications for the prevention and treatment of breast cancer.