Recording of Absorption Spectrum

Oct 13, 2025 by windailys.com 35 views

Determining Simultaneous Levels of Binary Mixture Chloramphenicol and Prednisolone in Cream Preparations with Ultraviolet Spectrophotometry Methods Mean Centering of Ratio Spectra (MCR)

Introduction

The analysis of binary mixtures of drugs in pharmaceutical preparations is a crucial aspect of quality control. Chloramphenicol and prednisolone are a combination of drugs widely available in pharmacies in the form of cream preparations. However, the overlapping nature of their spectra poses a significant challenge in determining their simultaneous levels. In this study, we employed the Mean Centering of Ratio Spectra (MCR) method in ultraviolet spectrophotometry to analyze the levels of chloramphenicol and prednisolone in cream preparations.

Research Purposes

The primary objective of this study was to determine the levels of chloramphenicol and prednisolone in cream preparations using the ultraviolet spectrophotometry method with MCR. The MCR method is particularly relevant in this context due to the overlapping nature of the spectra of these two active ingredients. The accurate determination of their levels is essential to ensure the quality and efficacy of the cream preparations.

Methodology

The sample used in this study consisted of two cream preparations, X and Y, each containing 20 mg of chloramphenicol and 2.5 mg of prednisolone per gram. Absolute ethanol was used as the solvent. The analysis process involved several steps, including:

Recording of Absorption Spectrum: Measuring the absorbance of the sample at a specific wavelength.
Making Spectrum Ratio: Calculating the ratio between the sample absorbance and the absorbance of the solvent.
Making MCR Spectrum: Using mathematical analysis to obtain a clearer spectrum.

After obtaining the spectrum, the study continued with the evaluation of the analytical characteristics of the method, including accuracy, precision, selectivity, and detection limits (LOD and LoQ).

Research Results

The results of the validation of chloramphenicol and prednisolone levels in cream X met all the specified requirements. The details of the validation results are as follows:

Chloramphenicol:
- Accuracy: 100.49%
- Precision: 0.57%
- LOD: 0.7318 μg/ml
- LoQ: 2,4392 μg/ml
Prednisolone:
- Accuracy: 99.93%
- Precision: 0.58%
- LOD: 0.4910 μg/ml
- LoQ: 1,6367 μg/ml

The levels of chloramphenicol in cream X were recorded at (99.80 ± 0.84)% and in cream Y at (98.69 ± 0.84)%. Meanwhile, the levels of prednisolone in cream X were (101.35 ± 1.08)% and in cream Y were (101.81 ± 0.69)%.

Conclusion

Based on the results of the study, it can be concluded that the ultraviolet spectrophotometry method with the effective MCR approach is a reliable method for determining the levels of chloramphenicol and prednisolone in cream preparations. This method meets the criteria for accuracy, precision, selectivity, and detection limits required. The use of MCR in spectrophotometric analysis not only increases accuracy but also provides solutions to the challenges faced by traditional techniques when dealing with compounds that have overlapping spectra. This makes this research contribute significantly to the field of pharmaceutical analysis.

Implications and Future Directions

The findings of this study have significant implications for the pharmaceutical industry. The use of MCR in spectrophotometric analysis can be applied to other binary mixtures of drugs, providing a reliable and accurate method for quality control. Future studies can focus on the application of MCR in other fields, such as environmental analysis and food safety. Additionally, the development of new mathematical models and software can further improve the accuracy and efficiency of MCR in spectrophotometric analysis.

Limitations and Recommendations

While this study demonstrates the effectiveness of MCR in determining the levels of chloramphenicol and prednisolone in cream preparations, there are some limitations to consider. The study was limited to two cream preparations, and further research is needed to validate the method for other types of pharmaceutical preparations. Additionally, the development of new mathematical models and software can further improve the accuracy and efficiency of MCR in spectrophotometric analysis.

Conclusion

In conclusion, this study demonstrates the effectiveness of the ultraviolet spectrophotometry method with MCR in determining the levels of chloramphenicol and prednisolone in cream preparations. The method meets the criteria for accuracy, precision, selectivity, and detection limits required, making it a reliable and accurate method for quality control. The use of MCR in spectrophotometric analysis provides solutions to the challenges faced by traditional techniques when dealing with compounds that have overlapping spectra, making this research contribute significantly to the field of pharmaceutical analysis.
Frequently Asked Questions (FAQs) about Determining Simultaneous Levels of Binary Mixture Chloramphenicol and Prednisolone in Cream Preparations with Ultraviolet Spectrophotometry Methods Mean Centering of Ratio Spectra (MCR)

Q: What is the Mean Centering of Ratio Spectra (MCR) method?

A: The MCR method is a mathematical approach used in ultraviolet spectrophotometry to analyze the levels of binary mixtures of drugs in pharmaceutical preparations. It involves the use of mathematical analysis to obtain a clearer spectrum, which helps to determine the levels of the two active ingredients.

Q: Why is the MCR method relevant in this study?

A: The MCR method is particularly relevant in this study because the spectra of chloramphenicol and prednisolone overlap, making it challenging to determine their simultaneous levels using traditional techniques. The MCR method provides a reliable and accurate method for quality control.

Q: What are the advantages of using the MCR method in spectrophotometric analysis?

A: The MCR method has several advantages, including increased accuracy, improved selectivity, and enhanced detection limits. It also provides solutions to the challenges faced by traditional techniques when dealing with compounds that have overlapping spectra.

Q: What are the limitations of the MCR method?

Q: Can the MCR method be applied to other binary mixtures of drugs?

A: Yes, the MCR method can be applied to other binary mixtures of drugs, providing a reliable and accurate method for quality control. However, further research is needed to validate the method for other types of pharmaceutical preparations.

Q: What are the implications of this study for the pharmaceutical industry?

A: The findings of this study have significant implications for the pharmaceutical industry. The use of MCR in spectrophotometric analysis can be applied to other binary mixtures of drugs, providing a reliable and accurate method for quality control.

Q: What are the future directions for this research?

A: Future studies can focus on the application of MCR in other fields, such as environmental analysis and food safety. Additionally, the development of new mathematical models and software can further improve the accuracy and efficiency of MCR in spectrophotometric analysis.

Q: What are the recommendations for further research?

A: Further research is needed to validate the MCR method for other types of pharmaceutical preparations. Additionally, the development of new mathematical models and software can further improve the accuracy and efficiency of MCR in spectrophotometric analysis.

Q: What are the potential applications of the MCR method in other fields?

A: The MCR method has potential applications in other fields, such as environmental analysis and food safety. It can be used to analyze the levels of pollutants in water and soil, as well as the levels of contaminants in food products.

Q: What are the benefits of using the MCR method in spectrophotometric analysis?

A: The MCR method has several benefits, including increased accuracy, improved selectivity, and enhanced detection limits. It also provides solutions to the challenges faced by traditional techniques when dealing with compounds that have overlapping spectra.

Q: Can the MCR method be used to analyze the levels of other compounds?

A: Yes, the MCR method can be used to analyze the levels of other compounds, including those with overlapping spectra. However, further research is needed to validate the method for other types of compounds.

Q: What are the potential limitations of the MCR method?

A: While the MCR method is a reliable and accurate method for quality control, it has some limitations. The study was limited to two cream preparations, and further research is needed to validate the method for other types of pharmaceutical preparations. Additionally, the development of new mathematical models and software can further improve the accuracy and efficiency of MCR in spectrophotometric analysis.