Double Divisor Ratio Derivative

Oct 13, 2025 by windailys.com 132 views

Determining Vitamin B1, Vitamin B6, and Vitamin B12 Levels in Ultraviolet Spectrophotometry Tablet Dosage with Double Divisor Divisor Spectra Derivative Method

Introduction

The quality of drug preparations is a crucial aspect of ensuring the efficacy and safety of medications. With the increasing complexity of modern pharmaceuticals, the need for accurate and efficient analysis methods has become more pressing. One such method is ultraviolet (UV) spectrophotometry, which has been widely used to determine the levels of various vitamins and compounds in drug preparations. In this study, we aim to develop a spectrophotometric method using the Double Divisor Ratio Spectra Derivative approach to determine the levels of vitamins B1, B6, and B12 in tablet dosage without the need for complicated separation processes.

Background

Most drugs on the market are a combination of several active ingredients, aiming to increase the therapeutic effects of each compound. One such combination is the co-administration of vitamins B1, B6, and B12, which is often found in the salut tablet preparation. To ensure the quality of these drug preparations, a simple and fast analysis method is needed. UV-VIS spectrophotometry is one of the potential choices to test the quality of drug preparations with a variety of vitamins B.

Objective

The primary objective of this study is to develop a spectrophotometric method with the Double Divisor Ratio Spectra Derivative approach to determine the levels of vitamins B1, B6, and B12 in tablet dosage without the need for complicated separation processes. This method is expected to be a more practical and efficient alternative compared to conventional methods that require complicated separation processes.

Methodology

This study uses the Double Divisor Ratio Spectra Derivative method with a spectrum derivative in the first derivative (Δλ 2) and uses methanol as a solvent. The method is validated through linearity parameters, accuracy, precision, detection limit (LOD), and quantitation limits (LOQ) to ensure its accuracy and reliability.

Results

The results of this study showed that the Double Divisor Ratio Spectra Derivative method was able to determine the levels of vitamins B1, B6, and B12 accurately. The levels of vitamins B1, B6, and B12 obtained respectively are (99,2796 ± 2,1806)%, (99,9997 ± 1,8169)%, and (97,9568 ± 2,2864)%. The validation test shows the correlation coefficient (R) value of 0.9998 for vitamin B1, -0.9997 for vitamin B6, and 0.999 for vitamin B12. The percentage of recovery for vitamins B1, B6, and B12 respectively is 99.82%, 100.56%, and 100.75%. The relative standard deviation (RSD) for the three vitamins is 1.23%, 1.22%, and 3.50%.

Discussion

The results of this study demonstrate the effectiveness of the Double Divisor Ratio Spectra Derivative method in determining the levels of vitamins B1, B6, and B12 in tablet preparations. This method is proven to meet the level requirements according to the Indonesian Pharmacopoeia (FI) edition VI and meets the validation requirements of the method. The advantages of this method include:

Practical and efficient: Does not require a complicated separation process, so it is more time-efficient and resource-effective.
Accurate: The results of determining the levels of vitamins B1, B6, and B12 showed high accuracy and in accordance with the requirements of the FI edition VI.
Ease of application: This method can be applied in a laboratory with equipment that is relatively simple and easy to obtain.

Conclusion

Based on the results of this study, it can be concluded that the Double Divisor Ratio Spectra Derivative method can be used to determine the levels of vitamins B1, B6, and B12 in tablet preparations. This method offers several advantages, including practicality, efficiency, accuracy, and ease of application. The development of this spectrophotometric method with the Double Divisor Ratio Derivative approach is a potential alternative in controlling the quality of drug preparations containing vitamins B1, B6, and B12.

Recommendations

This study recommends the use of the Double Divisor Ratio Spectra Derivative method in determining the levels of vitamins B1, B6, and B12 in tablet preparations. This method is a more practical and efficient alternative compared to conventional methods that require complicated separation processes. The results of this study demonstrate the effectiveness of this method in meeting the level requirements according to the Indonesian Pharmacopoeia (FI) edition VI and meeting the validation requirements of the method.

Future Directions

Future studies can build upon the findings of this study by exploring the application of the Double Divisor Ratio Spectra Derivative method in determining the levels of other vitamins and compounds in drug preparations. Additionally, the development of this method can be further improved by optimizing the parameters of the method, such as the solvent used and the wavelength of the spectrophotometer.

Limitations

This study has several limitations, including the use of a single solvent (methanol) and the limited number of samples analyzed. Future studies can address these limitations by exploring the use of different solvents and analyzing a larger number of samples.

Conclusion

In conclusion, this study demonstrates the effectiveness of the Double Divisor Ratio Spectra Derivative method in determining the levels of vitamins B1, B6, and B12 in tablet preparations. This method offers several advantages, including practicality, efficiency, accuracy, and ease of application. The development of this spectrophotometric method with the Double Divisor Ratio Derivative approach is a potential alternative in controlling the quality of drug preparations containing vitamins B1, B6, and B12.
Frequently Asked Questions (FAQs) about Determining Vitamin B1, Vitamin B6, and Vitamin B12 Levels in Ultraviolet Spectrophotometry Tablet Dosage with Double Divisor Divisor Spectra Derivative Method

Q: What is the Double Divisor Ratio Spectra Derivative method?

A: The Double Divisor Ratio Spectra Derivative method is a spectrophotometric method used to determine the levels of vitamins B1, B6, and B12 in tablet preparations. This method uses a spectrum derivative in the first derivative (Δλ 2) and methanol as a solvent.

Q: What are the advantages of the Double Divisor Ratio Spectra Derivative method?

A: The advantages of the Double Divisor Ratio Spectra Derivative method include:

Practical and efficient: Does not require a complicated separation process, so it is more time-efficient and resource-effective.
Accurate: The results of determining the levels of vitamins B1, B6, and B12 showed high accuracy and in accordance with the requirements of the FI edition VI.
Ease of application: This method can be applied in a laboratory with equipment that is relatively simple and easy to obtain.

Q: What are the limitations of the Double Divisor Ratio Spectra Derivative method?

A: The limitations of the Double Divisor Ratio Spectra Derivative method include:

Use of a single solvent (methanol): Future studies can explore the use of different solvents to improve the accuracy of the method.
Limited number of samples analyzed: Future studies can analyze a larger number of samples to improve the reliability of the method.

Q: Can the Double Divisor Ratio Spectra Derivative method be used to determine the levels of other vitamins and compounds in drug preparations?

A: Yes, the Double Divisor Ratio Spectra Derivative method can be used to determine the levels of other vitamins and compounds in drug preparations. Future studies can explore the application of this method in determining the levels of other vitamins and compounds.

Q: What are the future directions for the Double Divisor Ratio Spectra Derivative method?

A: Future studies can build upon the findings of this study by exploring the application of the Double Divisor Ratio Spectra Derivative method in determining the levels of other vitamins and compounds in drug preparations. Additionally, the development of this method can be further improved by optimizing the parameters of the method, such as the solvent used and the wavelength of the spectrophotometer.

Q: What are the recommendations for the use of the Double Divisor Ratio Spectra Derivative method?

A: This study recommends the use of the Double Divisor Ratio Spectra Derivative method in determining the levels of vitamins B1, B6, and B12 in tablet preparations. This method is a more practical and efficient alternative compared to conventional methods that require complicated separation processes.

Q: What are the implications of the Double Divisor Ratio Spectra Derivative method for the quality control of drug preparations?

A: The Double Divisor Ratio Spectra Derivative method has significant implications for the quality control of drug preparations. This method offers a more practical and efficient alternative compared to conventional methods that require complicated separation processes. The results of this study demonstrate the effectiveness of this method in meeting the level requirements according to the Indonesian Pharmacopoeia (FI) edition VI and meeting the validation requirements of the method.

Q: What are the future research directions for the Double Divisor Ratio Spectra Derivative method?

A: Future research directions for the Double Divisor Ratio Spectra Derivative method include:

Optimization of the parameters of the method: Future studies can optimize the parameters of the method, such as the solvent used and the wavelength of the spectrophotometer, to improve the accuracy and reliability of the method.
Application of the method in determining the levels of other vitamins and compounds: Future studies can explore the application of the Double Divisor Ratio Spectra Derivative method in determining the levels of other vitamins and compounds in drug preparations.
Development of new spectrophotometric methods: Future studies can develop new spectrophotometric methods that are more practical and efficient than the Double Divisor Ratio Spectra Derivative method.